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Lab Anim 1990;24:14-24
doi:10.1258/002367790780890329
© 1990 Laboratory Animals Limited

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A method for perfusion of the isolated rabbit liver through the portal vein and the hepatic artery in a recirculating system

G. W. Meijer, M. J. H. Geelen, H. Van Herck, J. W. M. A. Mullink, L. F. M. Van Zutphen and A. C. Beynen

Department of Laboratory Animal Science, PO Box 80.166, State University, The Netherlands; Laboratory of Veterinary Biochemistry, PO Box 80.176, State University, The Netherlands; Laboratory of Veterinary Biochemistry, PO Box 80.176, State University, The Netherlands; Department of Laboratory Animal Science, PO Box 80.166, State University, The Netherlands; Department of Veterinary Pathology, PO Box 80.158, State University, The Netherlands; Department of Laboratory Animal Science, PO Box 80.166, State University, The Netherlands; Department of Laboratory Animal Science, PO Box 80.166, State University, The Netherlands; Department of Laboratory Animal Science, PO Box 80.166, State University, The Netherlands

We describe a method for the isolation and recirculating perfusion of rabbit livers. Livers were perfused simultaneously through the portal vein and the hepatic artery. Precise descriptions of the surgical preparation, perfusion apparatus and perfusion method are given. The viability of rabbit livers was assessed with the use of physiological, biochemical and histological parameters. We conclude that the isolated perfused rabbit livers are sufficiently viable to study their short-term metabolism.

Key Words: RECIRCULATING LIVER PERFUSION • SURGICAL PREPARATION • PERFUSION APPARATUS • VIABILITY • RABBITS


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