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Lab Anim 1990;24:53-62
doi:10.1258/002367790780890374
© 1990 Laboratory Animals Limited
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Mouse strain differences in resident peritoneal cells: a flow cytometric analysis

Michael F. W. Festing, R. Legg, T. Eydmann and A. Brammall

Medical Research Council Toxicology Unit, Woodmansterne Road, Carshalton, Surrey, UK; Medical Research Council Toxicology Unit, Woodmansterne Road, Carshalton, Surrey, UK; Medical Research Council Toxicology Unit, Woodmansterne Road, Carshalton, Surrey, UK; Medical Research Council Experimental Embryology and Teratology Unit, St Georges Hospital Medical School, Tooting, UK

A flow-cytometric study of resident peritoneal cells among 8 mouse strains showed a more than twofold variation in the ratio of macrophages to macrophages plus lymphocytes, ranging from 27% in A/J to 62% in C57B/L10, with significant strain differences in a number of other cellular parameters. There was a particular deficiency of lymphocytes in strain CBA/N, which carries the xid mutation. Studies of the phagocytosis of fluorescent beads also revealed large differences in the number of beads taken up, ranging from 0·99 per cell in MFI to 1·64 per cell in BALB/c mice in a 20-min period. The total number of peritoneal cells collected also varied between strains, ranging from 2·75 x 106 in CBA/Ca to 5·85 x 106 in MFI. The total yield of macrophages per mouse ranged from 0·93 x 106 in A/J to 3·16 x 106 in C57BL/10. These differences should be taken into account when designing experiments which use resident peritoneal cells.

Key Words: INBRED MICE • MACROPHAGES • LYMPHOCYTES • PERITONEAL CELLS • FLOW CYTOMETRY


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