Laboratory Animals > Volume 25, Number 2 > Pp. 106-109

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Detection of mouse hepatitis virus antibody by protein A-ELISA in 6 prevalent inbred strains or outbred stocks of mice

Naoko Kagiyama, Akira Takakura, Kazuyuki Koyama, Eiji Terada and Yoshinori Sakurai

Department of Laboratory Animal Medicine, Central Institute for Experimental Animals, 1430 Nogawa, Miyamae, Kawasaki 216, Japan; Department of Laboratory Animal Medicine, Central Institute for Experimental Animals, 1430 Nogawa, Miyamae, Kawasaki 216, Japan; Taiho Pharmaceutical Co., Research Institute, 224-2 Hiraishi, Kawauchi, Tokushima, 771-01, Japan; Department of Laboratory Animal Science, School of Hygienic Sciences, Kitasato University, Kitasato, Sagamihara 228, Japan; Department of Laboratory Animal Science, School of Hygienic Sciences, Kitasato University, Kitasato, Sagamihara 228, Japan; Wakamoto Pharmaceutical Co., Ltd, 378 Kanade, Ohi-machi, Ashigarakami-gun, Kanagawa 258, Japan

Protein A was applied as a reagent for the secondary reaction in ELISA (protein A-ELISA). Mouse hepatitis virus antibody in 6 prevalent mouse strains or stocks reared in a MHV-contaminated room was effectively detected by protein A-ELISA, whereas significant strain differences in the antibody detection rate were demonstrated using the complement fixation test. C57BL/6 mice were particularly reactive in the protein A-ELISA test.

Key Words: MHV ANTIBODY • PROTEIN A-ELISA • MOUSE STRAIN DIFFERENCES

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