Laboratory Animals > Volume 25, Number 4 > Pp. 310-318

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Suppressive effect of nonviable Mycobacterium paratuberculosis on the delayed-type hypersensitivity reaction to sheep erythrocytes in mice

M. Kishima, Y. Yokomizo, I. Nonomura and N. Goto

National Institute of Animal Health, Kannondai 3-1-1, Tsukuba, lbaraki 305, Japan; National Institute of Animal Health, Kannondai 3-1-1, Tsukuba, lbaraki 305, Japan; National Institute of Animal Health, Kannondai 3-1-1, Tsukuba, lbaraki 305, Japan; National Institute of Animal Health, Kannondai 3-1-1, Tsukuba, lbaraki 305, Japan; Faculty of Agriculture, Kobe University, Kobe, Hyogo 657, Japan

The effect of nonviable Mycobacterium paratuberculosis on the delayed-type hypersensitivity reaction to sheep erythrocytes (SRBC) in mice was evaluated by means of delayed-type footpad swelling. Intraperitoneal (i.p.) injection with nonviable M. paratuberculosis into mice from 28 days before to 1 day after immunization with SRBC resulted in a significant suppression of footpad swelling to SRBC. The suppressive effect could be transferred by i.p. injection of spleen cells or peritoneal exudate cells from mice which had been pre-treated with nonviable M. paratuberculosis into non-treated recipient mice. The suppressive effect of spleen cells was retained even after passing them through a nylon wool column. The suppressive effect of spleen cells was abolished by treatment with anti-Thy 1·2 monoclonal antibody plus complement or anti-Lyt 2·2monoclonal antibody plus complement. However. treatment of spleen cells with anti-mouse globulin antiserum plus complement or anti-Lyt 1·2monoclonal antibody plus complement did not affect the. suppressive effect of spleen cells. Thesuppression of footpad swelling to SRBC induced by pre-treatment with nonviable M. paratuberculosis could be reversed by i.p. administration of cyclophosphamide. Serum antibody response to SRBC in mice was not affected by pre-treatment with nonviable M. paratuberculosis. These findings indicate that T cells appear to be involved in the suppression of delayed-type hypersensitivity reaction to SRBC in mice by pre-treatment with nonviable M. paratuberculosis.

Key Words: MICE • IMMUNOSUPPRESSION • MYCOBACTERIUM PARATUBERCULOSIS • DELAYED-TYPE HYPERSENSITIVITY

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