Laboratory Animals > Volume 25, Number 4 > Pp. 330-336

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Vitamin D, within its range of fluctuation in commercial rat diets, does not influence nephrocalcinogenesis in female rats

G. Marsman, F. J. H. Pastoor, J. N. J. J. Mathot, H. M. Theuns and A. C. Beynen

Department of Human Nutrition, Agricultural University, Wageningen, The Netherlands; Department of Laboratory Animal Science, State University, Utrecht, The Netherlands; Department of Laboratory Animal Science, State University, Utrecht, The Netherlands; Department of Laboratory Animal Science, State University, Utrecht, The Netherlands; Institute for Experimental Gerontology-TNO, Rijswijk, The Netherlands; Department of Human Nutrition, Agricultural University, Wageningen, The Netherlands; Department of Laboratory Animal Science, State University, Utrecht, The Netherlands

Massive, toxic doses of vitamin D have been shown to cause nephrocalcinosis in rats, but the effect of this vitamin within its range of fluctuation in commercial rat diets was unknown. Therefore, in two experiments with young female rats, the effect on nephrocalcinosis of a moderately increased level of vitamin D in the diet was studied, that is 5000 IU/kg versus the recommended concentration of 1000 IU/kg. This was done using purified diets with 0·5% (w/w) calcium and 0·04% magnesium containing either 0·2 or 0·06 phosphorus (P). Rats fed the diets containing 0·06%P showed severe kidney calcification compared to those fed the 0·2%-P diets. The level of vitamin D in the 0·2 and 0·6%-Pdiets did not affect kidney calcification. Bone density was increased after feeding diets containing 5000 instead of 1000 IV of vitamin D/kg. This study suggests that, within 28 days, a moderate increase of the amount of vitamin D in the diet has no influence on the development of kidney calcification. This in turn suggests that the variation in nephrocalcinosis severity and incidence seen in practice in rats fed different commercial diets is unlikely to be related to the different vitamin D concentrations in these diets. However, in rats fed such diets bone metabolism may be influenced differently.

Key Words: RATS • KIDNEY • DIET • VITAMIN D • NEPHROCALCINOSIS

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