Laboratory Animals > Volume 25, Number 4 > Pp. 342-347

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Toxicity of anticancer agents, growth and chemosensitivity of human tumour xenografts in a segregating stock of AF nude mice

Kohji Maruo, Reiko Emura, Yasuyuki Ohnishi, Sachio Endo, Yoshito Ueyama and Tatsuji Nomura

Central Institute for Experimental Animals, 1430 Nogawa, Miyamae, Kawasaki, Kanagawa 213, Japan; Department of Pathology, Tokai University, School of Medicine, Boseidai, Isehara, Kanagawa 259-11, Japan

An investigation of the usefulness of a segregating stock of nude mice [AF nude mice (AF-nu)] for screening anticancer agents was undertaken. The toxicity of anticancer agents, takes and growth rates of human tumour xenografts and chemosensitivities of xenografts in AF-nu were studied and compared with those in BALB/cA nude mice (BALB/cA-nu). The results showed differences in the pattern of mortalities of AF-nu and BALB/cA-nu administered a range of anticancer agents. Body weight changes in the two nude mouse strains differed in the case of 5-fluorouracil, but not for nimustine, adriamycin and vincristine. All tumours transplanted in AF-nu grew as in BALB/cA-nu. Growth rates of 2 xenografts (gastric cancer and glioblastoma) were not significantly different between the 2 nude mouse strains, but those of 2 lung tumour xenografts were significantly greater in AF-nu than those in BALB/cA-nu. There were no significant differences in chemosensitivities of human tumours in AF-nu and BALB/cA-nu (consistency rate as evaluated by our criteria was 88%). From these results, it is suggested that AF-nu are more suitable for anticancer agent screening and experimental chemotherapy of human tumour xenografts than BALB/cA-nu because of lower costs and high reproductive rate. Although they are genetically heterogeneous, sets of experimental animals sharing the same gene pool can be produced routinely.

Key Words: NUDE MOUSE • HUMAN TUMOUR XENOGRAFT • ANTICANCER AGENT • CHEMOTHERAPY

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