Laboratory Animals > Volume 29, Number 3 > Pp. 294-299

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Serological studies of Corynebacterium kutscheri and coryneform bacteria using an enzyme-linked immunosorbent assay (ELISA)

R. Boot, H. Thuis, R. Bakker and J. L. Veenema

Section of Laboratory Animal Microbiology, Laboratory for Parasitology and Mycology, National Institute of Public Health and Environmental Protection, PO Box 1, 3720 BA Bilthoven, the Netherlands; Section of Laboratory Animal Microbiology, Laboratory for Parasitology and Mycology, National Institute of Public Health and Environmental Protection, PO Box 1, 3720 BA Bilthoven, the Netherlands; Section of Laboratory Animal Microbiology, Laboratory for Parasitology and Mycology, National Institute of Public Health and Environmental Protection, PO Box 1, 3720 BA Bilthoven, the Netherlands; Section of Laboratory Animal Microbiology, Laboratory for Parasitology and Mycology, National Institute of Public Health and Environmental Protection, PO Box 1, 3720 BA Bilthoven, the Netherlands

An enzyme-linked immunosorbent assay (ELISA) to measure Corynebacterium kutscheri antibodies in mice and rats was developed. Seven c. kutscheri isolates showed considerable serological relationship, but Japanese isolates differed from the British isolates. The ELISA appeared specific since c. kutscheri antigen did not react with antisera against 8 heterologous coryneform species. Antibodies to c. kutscheri were to a limited extent absorbed by autologous and homologous antigen, but not at all by the heterologous coryneform species.

In naturally infected wild Rattus norvegicus and laboratory NA rats, the ELISA demonstrated high ODs to c. kutscheri.

Key Words: CORYNEBACTERIUM KUTSCHERI • CORYNEFORM BACTERIA • SEROLOGY • ELISA • MOUSE • RAT

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