Laboratory Animals > Volume 29, Number 3 > Pp. 307-313

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An enzyme-linked immunosorbent assay (ELISA) for monitoring rodent colonies for Pasteurella pneumotropica antibodies

R. Boot, H. C. W. Thuis, J. L. Veenema and R. G. H. Bakker

Section of laboratory Animal Microbiology, laboratory for Parasitology and Mycology, National Institute of Public Health and Environmental Protection, P.O. Box 3720 BA Bilthoven, The Netherlands; Section of laboratory Animal Microbiology, laboratory for Parasitology and Mycology, National Institute of Public Health and Environmental Protection, P.O. Box 3720 BA Bilthoven, The Netherlands; Section of laboratory Animal Microbiology, laboratory for Parasitology and Mycology, National Institute of Public Health and Environmental Protection, P.O. Box 3720 BA Bilthoven, The Netherlands; Section of laboratory Animal Microbiology, laboratory for Parasitology and Mycology, National Institute of Public Health and Environmental Protection, P.O. Box 3720 BA Bilthoven, The Netherlands

An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of Pasteurella pneumotropica antibodies in the sera of rats} mice, hamsters and Mastomys. P. pneumotropica from mice and rats showed cross-reactivity. The ELISA using P. pneumotropica NCTC 8284 detected more infected animals than selective culture in groups of rodents from which P. pneumotropica, Haemophilus sp and/or Actinobacillus sp were cultured. Cross reactivity between P. pneumotropica NCTC 8284 and haemophilus and actinobacillus isolates were not studied.

Key Words: ACTINOBACILLUS • HAEMOPHILUS • PASTEURELLA • SEROLOGY • ELISA • CULTURE • RODENTS

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