Laboratory Animals > Volume 32, Number 1 > Pp. 86-94

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Detection of endogenous retrovirus antigens in NOD mouse pancreatic β-cells

H. Tsumura, M. Miyazawa, S. Ogawa, J. Z. Wang, Y. Ito and K. Shimura

Institute of Laboratory Animals, Mie University School of Medicine, 2–174 Edobashi, Tsu, Mie 514, Japan; Department of Pathology, Tohoku University School of Medicine, 2-1 Seiryo, Aoba, Sendai 980-77, Japan; Institute of Laboratory Animals, Mie University School of Medicine, 2–174 Edobashi, Tsu, Mie 514, Japan; Department of Microbiology, Mie University School of Medicine, 2–174 Edobashi, Tsu, Mie 514, Japan; Institute of Laboratory Animals, Mie University School of Medicine, 2–174 Edobashi, Tsu, Mie 514, Japan

We characterized C-type retroviruses expressed in the pancreatic β-cells of non-obese diabetic (NOD) mice by immunohistochemical techniques and by inhibiting the production of viral particles using antisense oligonucleotides. Some cells in the pancreatic islets from both NOD and diabetes-resistant NOD-related mice (NON) reacted with a monoclonal antibody directed against the envelope protein(s) of polytropic viruses. On the other hand, NOD islet cells also showed strong immunoreactivity with an anti-gag protein monoclonal antibody and another anti-envelope protein(s) monoclonal antibody that is specific for xenotropic viruses. In antisense oligodeoxynucleotide inhibition assays, a xenotropic virus-specific phosphorothionate antisense oligodeoxynucleotide significantly inhibited the occurrence of C-type virus particles in NOD mouse islet β-cells. Therefore, C-type retrovirus-like particles expressed in NOD mouse pancreatic β-cells were considered to be endogenous xenotropic virus. The expression of the xenotropic viral genome may be involved in the pathogenesis of the diabetic syndrome in NOD mice.

Key Words: NOD MOUSE • RETROVIRUS

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