Laboratory Animals > Volume 42, Number 2 > Pp. 193-203

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A simplified tumour model established via Epstein-Barr virus-encoded, nasopharyngeal carcinoma-derived oncogene latent membrane protein 1 in immunocompetent mice

Kai-Ping N Chow ^* , C C Wu ^*, H Y Chang , C Chang  and Y S Chang 

^* Department of Microbiology and Immunology; Graduate Institute of Basic Medical Sciences, School of Medicine, Chang-Gung University, Kwei-shan, Taoyuan 333; Institute of Molecular Medicine, National Tsing Hua University, Hsin-chu 300; Functional and Micro-Magnetic Resonance Imaging Center, Institute of Biomedical Sciences, Academic Sinica, Taipei 11529, Taiwan, Republic of China

Correspondence: Kai-Ping N. Chow, PhD, Department of Microbiology and Immunology, School of Medicine, Chang-Gung University, No. 259, Wen-Hwa First Road, Kwei-shan, Taoyuan 333, Taiwan, Republic of China. Email: kpc{at}mail.cgu.edu.tw

The expression and immune modulation of Epstein-Barr virus-encoded oncogene latent membrane protein 1 (N-LMP1) is essential in the pathogenesis of nasopharyngeal carcinoma. In previous studies, cell transformation has been induced by the expression of EBV-encoded N-LMP1 in non-tumour BALB/c-3T3 cells and these cells have then been used to form tumours in T-cell-deficient nude mice. However, studies using this model have been limited by the lack of a competent immune system. To facilitate the study of immune components in N-LMP1-driven oncogenesis, we herein developed a simplified N-LMP1-derived tumour model in immunocompetent mice. Cell transformation was induced by the expression of N-LMP1 in BALB/c-3T3 cells, and these transformants were used to induce oncogenesis in BALB/c mice. In contrast to the 100% successful tumour-induction rate in nude mice treated with monodispersed transformed cells, the tumour incidence in BALB/c mice was only 5–36%. However, the transplantation of tumour fragments into BALB/c mice yielded a reproducible tumour-induction rate of >85%, which is acceptable for most of the research needs. This novel model of N-LMP1-directed oncogenesis in an immunocompetent environment may serve as an important platform for the future assessment of N-LMP1-targeted tumour therapies.

Key Words: Nasopharyngeal carcinoma • Epstein-Barr virus (EBV) • oncogene latent membrane protein 1 (N-LMP1) • tumour mouse model

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