Laboratory Animals > Laboratory Animals Online First > 10.1258/la.2009.009003

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Original Article

An automatic video instillator for intratracheal instillation in the rat

Jin-Sung Kim ^1 4 , Bae Lee ², In-Chul Hwang ², Young-Su Yang ³, Mi-Jin Yang ³ and Chang-Woo Song ³

¹ Department of Non-clinical Studies, Korea Institute of Toxicology, Korea Research Institute of Chemical Technology, 19 ShinSeong-Ro, YuSeong, DeaJeon 305-343, Korea; ² Doobae System Co. Ltd, 518-1 Sungnae-Dong, Gangdong-Gu, Seoul 134-031, Korea; ³ Division of Inhalation Toxicology, Korea Institute of Toxicology, Korea Research Institute of Chemical Technology, 1051 ShinJeong-Dong, JeongEup-Si, Jeollabuk-Do 580-185, Korea; ⁴ Current address: Clinical Trial Center, Chonbuk National University Hospital, 634-18 Geumam-Dong, Deokjin-Gu, Jeonju-Si, Jeollabuk-Do 561-712, Korea

Corresponding author: Jin-Sung Kim. Email: kjs{at}ctcf2.com

Intratracheal instillation (ITI) of a test compound is an alternative method to inhalation methods that require complex aerosol generation, exposure chambers and airflow monitoring instruments for exposing the lungs of animals to a test compound. For ITI in the rat, a laryngoscope is generally used for endotracheal intubation, and the procedure is difficult to perform. Therefore, we designed and constructed an automatic video instillator (AVI) for the accurate delivery of a dose of a test compound into the trachea of rats. The device has a videocamera probe for image guidance, and a liquid-crystal display for image display. These two items are used to visualize the larynx and trachea for intratracheal insertion of the tubing, and for placing the tip of the instillation tubing beyond the vocal cords for ITI of the test compound. After a 2 h training session on the use of the AVI in an anaesthetized rat, we assessed the utility of the device by ITI of 0.25% (w/v) solution of Evans Blue dye into the lungs of 30 isoflurane-anaesthetized rats. Necropsy examinations were performed on 20 rats immediately after the completion of the procedure, and on 10 rats three days after the procedure. Based on the results of these examinations, we concluded that the device could be used for rapid, reproducible and successful ITI of a test compound into the lungs of a rat by one operator.

Key Words: Intratracheal instillation • pulmonary toxicology • instillator

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