Laboratory Animals > Laboratory Animals Online First > 10.1258/la.2009.009042

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Original Article

Kinetics of ₂-macroglobulin and ₁-acid glycoprotein in rats subjected to repeated acute inflammatory stimulation

Toshio Honjo ¹, Takashi Kuribayashi ², Mariko Matsumoto ², Shunsuke Yamazaki ³ and Shizuo Yamamoto ² 

¹ Seminar on Environment; ² Laboratory of Immunology, School of Life and Environmental Science, Azabu University, Fuchinobe 1-17-71, Sagamihara, Kanagawa 229-8501, Japan; ³ Laboratory of Physiology, Department of Food and Nutrition, Kamakura Women's University, Ofuna 6-7-3, Kamakura, Kanagawa 247-8512, Japan

Corresponding author: S Yamamoto. E-mail: yamamoto{at}azabu-u.ac.jp

The kinetics of ₂-macroglobulin (2M) and ₁-acid glycoprotein (AAG) in rats repeatedly stimulated with intramuscular injections of turpentine oil at doses 0.05 and 0.4 mL/rat were investigated. Mean serum levels of 2M peaked at 48 h after the first turpentine oil injection, reaching 1.74 and 2.36 mg/mL at 0.05 and 0.4 mL/rat, respectively. AAG peaks were also observed at 48 h after injection, and the mean values were 2.02 and 2.53 mg/mL, respectively. These peak values of 2M and AAG differed significantly between the 0.05 and 0.4 mL/rat injection groups. Mean serum levels of interleukin-6 (IL-6) at 0.05 mL/rat were 52.61 pg/mL at 12 h, 48.86 pg/mL at 36 h and 81.93 pg/mL at 84 h after the first injection. Mean IL-6 serum levels at 0.4 mL/rat were 215.24 pg/mL at 12 h, 56.33 pg/mL at 36 h and 39.25 pg/mL at 84 h after the first injection. Mean serum levels of cytokine-induced chemoattractant-1 (CINC-1) at a dose of 0.05 mL/rat were 5.70 ng/mL at 12 h, 5.58 ng/mL at 36 h and 4.58 ng/mL at 84 h after the first injection. Mean serum levels of CINC-1 after injection at 0.4 mL/rat were 11.57 ng/mL at 12 h, 4.68 ng/mL at 36 h and 4.42 ng/mL at 84 h. Serum levels of IL-6 differed significantly at 12, 24, 72 and 84 h, while those of CINC-1 differed significantly at 12, 24, 48 and 96 h between the 0.05 and 0.4 mL/rat injection groups. Differences in peak serum levels in the 0.05 and 0.4 mL/rat groups were attributed to differences in the production of IL-6 and CINC-1, which are thought to contribute to 2M and AAG production.

Key Words: ₂-macroglobulin • ₁-acid glycoprotein • rats • cytokines • repeated injection

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