Laboratory Animals > Volume 42, Number 4 > Pp. 489-494

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Collection and cryopreservation of preimplantation embryos of Cavia porcellus

M M Dorsch , S Glage and H J Hedrich

Institute for Laboratory Animal Science, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany

View larger version (69K): [in this window] [in a new window]   Figure 1 Frozen-thawed guineapig embryos at magnification x80. Cryoprotectant used was 1.5 mmol/L dimethyl sulphoxide. Embryos have been collected on day 2.5 of pregnancy after superovulation. (a) Normal light illumination, (b) Same embryos as in Figure 1a after fluorescien diacetate A staining. An ultraviolet filter for green fluorescence at 546 nm was used. Viable blastomers show green fluorescence

 

View larger version (70K): [in this window] [in a new window]   Figure 2 Frozen-thawed guineapig embryos at magnification x80. Cryoprotectant used was 1.5 mmol/L propanediol. Embryos have been collected on day 2.5 of pregnancy after superovulation. (a) Normal light illumination, (b) Same embryos as in Figure 2a after fluorescein diacetate staining. An ultraviolet filter for green fluorescence at 546 nm was used. Viable blastomers show green fluorescence

 

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