Laboratory Animals > Volume 42, Number 4 > Pp. 495-504

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Quantitative rat liver function test by galactose single point method

T H Young ^* , H S Tang , Y C Chao , H S Lee , C H Hsiong ^**, L H Pao ^** and O Y P Hu ^**  

^* Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan, ROC; Division of Gastroenterology, Department of Internal Medicine, Cardinal Tien Hospital, Fu Jen Catholic University, Taipei, Taiwan, ROC; Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC; Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC; ^** School of Pharmacy, National Defense Medical Center, Taipei, Taiwan, ROC; Department of Research and Development, National Defense Medical Center, 161 Minchuan East Road, Sector 6, Taipei, Taiwan 114, ROC

View larger version (21K): [in this window] [in a new window]   Figure 1 The mean (±SD) venous blood concentration–time profile of galactose after quick intravenous injection of 0.5 g/kg galactose in control (n = 8) and carbon tetrachloride (CCl4) treated rats (n = 8). The difference of galactose blood concentrations at 45, 60 and 90 min after infusion show highly significant differences between these two groups. It peaked at 60 min and reduced at 90 min (#P < 0.01, *P < 0.001, P < 0.005)

 

View larger version (9K): [in this window] [in a new window]   Figure 2 Male Sprague-Dawley rats were treated with carbon tetrachloride (CCl4) (1 mL/kg, intraperitoneally). Plasma was collected for the analysis of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and galactose single point (GSP) evaluation 24 h later. #P < 0.003, *P < 0.001 for CCl4 versus control

 

View larger version (160K): [in this window] [in a new window]   Figure 3 Photomicrography of liver sections from rats in the control group (A and D), isoniazid (INH) group (B and E) and INH-bis-p-nitrophenyl phosphate (INH-BNPP) treated group (C and F). (A) Normal morphology of liver tissue in control rats. (B) Hepatocellular disintegration and the vacuolation in the pericentral vein (V) area in INH-treated rats (haematoxylin & eosin stain, original magnification x400). Electron microscopic examination of the rat liver revealed increased proliferation of rough endoplasmic reticulum (rER) (E) compared with normal control (D) (original magnification x16,000). Histological section of the liver tissue of INH-BNPP-treated rats (C and F) showed similar patterns to the normal control (Nu: nucleus; Mito: mitochondria)

 

View larger version (10K): [in this window] [in a new window]   Figure 4 Effects of isoniazid (INH) and INH-bis-p-nitrophenyl phosphate (INH-BNPP) treatments for 21 days on serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities (bottom) and average galactose single point (GSP) values (top) in rats. *P < 0.001 for INH group versus the control or INH-BNPP group; #P < 0.05 for INH-BNPP versus the control group

 

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