Laboratory Animals > Laboratory Animals Online First > 10.1258/la.2007.007011

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Paper

Collection and cryopreservation of preimplantation embryos of Cavia porcellus

M M Dorsch , S Glage and H J Hedrich

Institute for Laboratory Animal Science, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany

Correspondence: M M Dorsch. Email: dorsch.martina{at}mh-hannover.de

Individual differences and a rather long-lasting reproductive cycle, as well as the relatively small number of oocytes that mature during one reproductive cycle makes it difficult to establish a cryopreserved stock of preimplantation embryos of the guineapig (Cavia porcellus) when compared with other laboratory rodents. Only a few data for superovulation protocols that can be used for routine laboratory use in guineapigs are available. However, a huge number of different strains exist for many purposes and the establishment of a frozen repository makes sense. Here, we describe the successful freezing of preimplatation embryos of the strain 2BS with a two-step freezing protocol in a freezing medium containing 1,2-propanediol as cryoprotectant. Human menopausal gonodotrophin induced superovulation in the embryo donors.

Key Words: Guineapig • cryopreservation • preimplantation embryo

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